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Postdoctoral Fellow
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BE-Biological Systems & Eng
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85812 Requisition #
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Berkeley Lab’s Biological Systems & Engineering Division has an opening for a Postdoctoral Scholar. The Biological Systems & Engineering Division of Berkeley Lab has an exciting opening in the Bioengineering and Biomedical Sciences Department for a Biochemistry Postdoctoral Scholar to join a team that focuses on elucidating the molecular mechanisms that underlie aging and their relationship to genome instability and cancer.  The project is part of a large multi-institutional Program funded by the National Institute on Aging of NIH. Under the general direction of the P.I., the Postdoc will conduct independent basic research in molecular and cell biology and biochemistry and will use human and rodent cell systems to address relationships between DNA repair, genome stability, cancer and aging, particularly with regard to the roles of multi-component protein complexes. The project involves close collaborations with The Buck Institute for Research on Aging (Novato, CA) and the Erasmus Medical Center (Rotterdam).

Aging is intimately intertwined with genome maintenance. Replication and transcription impose special demands, requiring the coordinated contribution of multiple DNA repair processes to maintain genomic integrity. Breakdown in any of these systems or in their coordination can cause replication fork collapse and DNA double-strand breaks (DSBs), transcriptional stress, and genomic instability – a hallmark of aging. The Postdoc will join a project that is designed to provide molecular mechanistic understanding of how particular key genome maintenance proteins prevent aging pathologies. Research will be conducted to test the overarching hypothesis that integration and coordination of multiple DNA repair processes with each other and with the replication and transcription machineries maintains damage-free templates for transcription with avoidance of transcriptional stress, which is proposed to be a novel major contributor to aging.  


What You Will Do:

  • Design and construct mammalian expression vectors for recombinant proteins and reporter constructs; transfect into human or mouse cells for analysis of functional endpoints.

  • Knockdown selected proteins in mammalian cells using siRNA or shRNA; mutate and/or tag genomic copies of mammalian DNA repair genes in situ using CRISPR/Cas9 technologies.

  • Investigate and characterize protein-protein interactions and regulation of protein function by assembly into complexes and/or by post-translational modifications.

  • Use ChIP-qPCR to analyze recruitment of specific proteins to defined DSBs in particular genomic contexts using a novel human cell system and assay repair kinetics of these DSBs after knockdown of specific proteins.

  • Characterize the effects of alterations in key DNA repair proteins on cell growth, viability, transcriptional stress, R-loop formation, and cellular responses to DNA damage, especially cell fate outcomes (cellular senescence, the senescence-associated secretory phenotype, and induction of apoptosis).

  • Analyze data and prepare results for presentation; present research data at group meetings and at conferences as appropriate.

  • Prepare manuscripts for publication in peer-reviewed scientific journals.

  • Maintain an accurate and detailed laboratory notebook of all experiments performed. Provide sufficient information to ensure that the work could be reproduced

  • Interact productively with other members of the P.I.’s laboratory and with collaborating laboratories to further the scientific goals of the research program.


Additional Responsibilities as needed:

  • Participate in group meetings, seminars, and workshops.

  • Provide guidance to undergraduate students.

  • Receive training in, and assist the PI with, preparation of grant applications and progress reports.


What is Required:

  • Ph.D. within the last four years in molecular biology, biochemistry, genetics, or related field.

  • Experience in protein biochemistry including quantitative western blot analysis, biochemical fractionation of cells, protein-protein and protein-DNA interaction studies, and a range of enzymatic assays.

  • Experience in ChIP and qPCR analysis.

  • Extensive experience in mammalian cell culture, transfection, cell biological assays including immunofluorescence, and reporter assays.

  • Demonstrated ability to design and carry out independent research, with track record of publishing in the peer-reviewed scientific literature.

  • Strong organizational skills.

  • Well-developed interpersonal and communication skills, with proven ability to communicate effectively both orally and in writing.

  • Demonstrated ability to work under PI guidance and with collaborators and to interact effectively with other technical and scientific staff members of a research team, as well as to conduct experiments independently.


Additional Desired Qualifications:

  • Background and previous research experience in molecular mechanisms of DNA repair and maintenance of genome stability.

  • Previous research experience in effects of DNA damage on transcription and/or replication.

  • Background and interest in cellular senescence and aging.


The posting shall remain open until the position is filled.


Notes:

  • This is a full time, 1 year,  postdoctoral appointment with the possibility of renewal based upon satisfactory job performance, continuing availability of funds and ongoing operational needs. You must have less than 4 years paid postdoctoral experience. Salary for Postdoctoral positions depends on years of experience post-degree.

  • Full-time, M-F, exempt (monthly paid) from overtime pay.

  • This position is represented by a union for collective bargaining purposes.

  • Salary will be predetermined based on postdoctoral step rates.

  • This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.

  • Work will be primarily performed at West Berkeley Biocenter (Potter St.) — Bldg. 977, 717 Potter St., Berkeley, CA.


Berkeley Lab (LBNL) addresses the world’s most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab’s scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy’s Office of Science.


Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4.  Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."


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Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."

 

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